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Heatshockisperformedat42°Cfor30secondsasappropriateforthebacterialstrainandDNAused.Forsmallervolumesofcellsinsmallertubes,theheat ...
Withchemicaltransformation,chemicallycompetentcellsaremixedwithplasmidDNAandbrieflyexposedtoanelevatedtemperature,aprocessknownasheatshock ...
2021年12月2日—HeatshocktransformationisthemostusedmethodofincorporatingrecombinantvectorsintoE.coli.Inthisarticle,theprotocolfor ...
HeatshocktransformationisthemostusedmethodofincorporatingrecombinantvectorsintoE.coli.Inthisarticle,theprotocolfortransformationis ...
由MRahimzadeh著作·2016·被引用65次—Abstract.CaCl2treatmentfollowedbyheatshockisthemostcommonmethodforartificialtransformation.Here,thecellsweretransformedusingCaCl2treatment ...
由MRahimzadeh著作·2016·被引用39次—CaCl2treatmentfollowedbyheatshockisthemostcommonmethodforartificialtransformation.Here,thecellsweretransformedusingCaCl2treatment ...