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ThisIHCprotocolprovidesabasicguideforthefixation,microtomesectioning,andstainingofparaffin-embeddedtissuesamples.Eachinvestigatormust ...
ThisIHCprotocolprovidesabasicguideforthefixation,microtomesectioning,andstainingofparaffin-embeddedtissuesamples.Eachinvestigatormust ...
Staining.WashsectionsindH2Othreetimesfor5minuteseach.Incubatesectionsin3%hydrogenperoxidefor10minutes.WashsectionsindH2Otwicefor5 ...
Stainparaffin-embedded,frozen,andfree-floatingsectionswithourIHCstainingprotocol.
H2O2suppressesendogenousperoxidaseactivitytoreducebackgroundstaining.Checkforthepresenceofendogenousperoxidasesbyincubatingatissueslide.
Immunohistochemicalstainingisaccomplishedwithantibodiesthatrecognizethetargetprotein.Sinceantibodiesarehighlyspecific,theantibodywillbind ...
Immunohistochemicalstainingisaccomplishedwithantibodiesthatrecognizethetargetprotein.Sinceantibodiesarehighlyspecific,theantibodywillbind ...
Thisguideprovidesanintroductiontothetechniques,protocols,andtroubleshootingmethodsthatleadtoasuccessfulimmunohistochemistry(IHC)or ...
由SWKim著作·2016·被引用257次—Immunohistochemistry(IHC)isanimportantauxiliarymethodforpathologistsinroutinediagnosticworkaswellasinbasicandclinicalresearchincluding ...
ProtocolDatabase·BlockingofUnwantedNon-specificStaining·Immunoenzyme(HRP)Method·Immunoenzyme(AP)Method·ImmunofluorescenceMethod·StandardABC( ...